Content

1. Theory of light, light matter interaction, with an emphasis on fluorescence
2. The Light Microscope: history and evolution, components of a compound microscope

Light microscopy
- The optical components of compound microscope and their functions/operations
- The physics of light and glass
- Forming and maintaining optical resolution with these lenses
- Proper(Kohler) bright field illumination
- Principles of sample preparation for light microscopy
- Theory of image formation, diffraction-limited resolution
- Optical aberrations and their correction
- Illumination and image forming techniques:
     * Brightfield Optics
     * Oblique Illumination Techniques
     * Imaging of Phase Objects
     * Polarization Optics
     * Fluorescence Microscopy
     * Probes for fluorescence microscopy
     * Confocal Microscopy
     * Single molecule microscopy and applications in biology
     * FCS and applications in biology, FRET and its applications
-  Labeling and labeling strategies
-  DNA sequencing with emphasis on single molecule optical approaches
-  Non-linear microscopy: Multi-photon excitation, Second Harmonic Imaging, different Raman microscopy schemes
  - New microscopies to improve resolution: STED, structured light microscopy, PALM/STORM, SOFI, expansion microscopy and eventual new modalities
-  Image recording, processing, and analysis

Light Microscopy selected readings:
Murphy, D.B., Fundamentals of Light Microscopy and Electronic Imaging. 2001, New York: John Wiley & Sons, Inc.
Molecular Expressions web site at the Florida State University

Course material

Slides/handouts

Recommended papers, recommended websites

Books

Format: more information

Guest lecture - Presentation

Content

See content lecture

Course material

Announcements and handouts distributed via Toledo

Format: more information

• Exercises aiming for deepening insight in the theoretical aspects of the course
• Hands-on experience with microscopy
• A presentation and discussion of a recent publication related to microscopy. The students can select the publication from a list provided in Toledo.

• If, for reasons of force majeure, the faculty decides that the hands-on microcopy sessions cannot be organized, they will be replaced by recorded demonstration sessions (most probably  Blackboard collaborate). The impact of this decision will be explained on Toledo. The assessment of the course will then be based on the remaining components (exam and presentation, respectively 1-16/16 and 1-4/4)