Bioinformatics and Genome Technology (B-KUL-I0P49A)
Aims
To extend knowledge on Gene Technology towards a genome scale, aimed at the experimental analysis and generation of genetic information at different molecular-biological levels.
The student gains insight into the current technologies available to study the different molecular-biological levels (Genome/transcriptome/proteome/interactome). The student can reproduce these, evaluate their applications and critically evaluate them for specific applications. This is achieved by specific examples, as provided by the instructors.
Based on the complexity of the different levels (DNA/RNA/protein/metabolites), the student knows and understands high-throughput techniques. The student knows and understands the current identification approaches (Sequence analysis/microarray, mass spectrometry) and can analyse and interpret the obtained biological information, based on bioinformatic and experimental approaches.
Previous knowledge
Knowledge of statistics, a mathematical basis of matrix calculus, basic knowledge on bioinformatics (DNA databases and their use), knowledge on microbiology, biochemistry and gene technology.
Order of Enrolment
This course unit is a prerequisite for taking the following course units:
I0P52A : Geïntegreerd practicum (GIP): moleculaire biotechnologie (No longer offered this academic year)
I0I31A : Integrated Practicals of Molecular Biotechnology
Identical courses
This course is identical to the following courses:
I0U28B : Omics Techniques and Data Analysis
Is included in these courses of study
- Master of Statistics and Data Science (on campus) (Leuven) (Statistics and Data Science for Biometrics) 120 ects.
- Courses for Exchange Students Faculty of Bioscience Engineering (Leuven)
- Master of Bioscience Engineering: Cellular and Genetic Engineering (Leuven) 120 ects.
Activities
2 ects. Genome Analysis (B-KUL-I0V62a)
Content
- Overview of genome complexity and genome mapping approaches
- High throughput sequencing analysis techniques
- Genome scale chemical synthesis approaches for nucleic acids
- Bioinformatic analysis of genomes
- Genome annotation
- Comparative and metagenome analysis
Course material
Handouts and notes
Is also included in other courses
1 ects. Transcriptome Analysis (B-KUL-I0V63a)
Content
- Techniques in transcriptome analysis
- Bioinformatic analysis of transcriptome data
- Related biostatistics
Course material
Course text / slides
Handouts and notes
Is also included in other courses
2 ects. Proteome and Metabolome Analysis (B-KUL-I0V64a)
Content
- Protein expression and analysis
- Proteome analysis approaches
- Principles of mass spectrometry
- Peptidomics
- Metabolomics
- Bioinformatical analysis of proteome data
- Examples and applications
Course material
Course notes / slides
Handouts and notes
Is also included in other courses
1 ects. Interaction Analysis of Biomolecules (B-KUL-I0V65a)
Content
- In vitro approaches to interactomics
- In vivo approaches to interactomics
- Examples and applications
Course material
Course tekst / slides
Handouts and notes
Evaluation
Evaluation: Bioinformatics and Genome Technology (B-KUL-I2P49a)
Explanation
- Open domain questions which assess knowledge, understanding, analysis and synthesis of the course materia
- Technique questions: define and illustrate specific techniques (knowledge, understanding and analysis)
- Definition questions which require synthesis skills
- Exercises during the exam will assess the critical evaluation and choice of specific techniques for defined applications