Required in stage
Optional in stage
Second term
Both terms
Not organised
This year
Next year
Alternating years
Prerequisites
Identical coursesBio-Molecular Interactions: Methods and Applications (B-KUL-H0A32A)
English
First term
Cannot be taken as part of an examination contractCannot be taken as part of a credit contract
N. 
Université Grenoble AlpesAims
We present techniques for the characterization of bio-molecular interactions as well as examples of applications of these molecules and their interactions. The lectures focus on spectroscopic methods (absorbance, fluorescence, light-scattering, …), but also includes thermodynamic measurements (calorimetry) and global techniques (proteomics, mass spectroscopy, …). Most of these methods probe interactions in solution. We also present the extension of these bulk techniques to the characterization of single-molecules, including optical tweezers, magnetic nanoparticles, biological nanopores, etc..
Previous knowledge
Optics, thermodynamics of solutions.
Is included in these courses of study
- Erasmus Mundus Master of Science in Nanoscience and Nanotechnology (Leuven et al) 120 ects.
- Erasmus Mundus Master of Science in Nanoscience and Nanotechnology (Leuven et al) (Option Bionanotechnology (UGA Grenoble)) 120 ects.
Activities
3 ects. Bio-Molecular Interactions: Methods and Applications (B-KUL-H0A32a)
EnglishFormat: PracticalFirst termN. Content
Introduction of the bio-molecular interaction and illustration with examples from the literature as well as applications in nanobiology and nano-biotechnology
- Presentation of the optical techniques:
- theoretical and practical basis
- Absorbance, fluorescence, fluorescence correlation, fluorescence polarization, fluorescence lifetime, Raman spectroscopy, etc.
- Micro-calorimetry: differential scanning calorimetry, isothermal titration calorimetry, ...
- Proteomics: global analysis of the contents of cells
- Single molecule techniques: assembly of DNA-origami structures, single molecule measurements (fluorescence, …), single molecule manipulations (optical tweezers, magnetic nanoparticles, …)
Practical training associated with the course:
- Optical tweezers for measuring Brownian motion
- Microcalorimetry for the characterization of DNA-origami
Course material
Text book, articles and literature, syllabus, slides.