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Identical coursesGenome, Proteome and Metabolome Analysis (B-KUL-G0R70A)
Temmerman Liesbet (coordinator) | Temmerman Liesbet | Van Belleghem Steven | N. Aims
The student
- has knowledge and insight in the “omics” technologies and their basic principles, which are currently applied in biological and biochemical research, in particular in genomics, transcriptomics, proteomics and metabolomics;
- is able to critiaclly reflect on the different omics techniques and their potential paractical applications;
- is able to process, analyse and interprete experimental data obtained by omics approaches using advanced software tools;
- is able to explain the omics techniques mentioned in a given research paper, as well as to explain the obtained results and deduced conclusions;
- is able to propose specific omics experiments to solve a given biological research question.
Previous knowledge
Basic knowledge of molecular biology, gene technology, biochemistry and bio-informatics
Identical courses
This course is identical to the following courses:
G0G57A : Genoom-, proteoom- en metaboloomanalyse
Is included in these courses of study
- Master of Biophysics, Biochemistry and Biotechnology (Leuven) (Specialisation: Biochemistry and Biotechnology) 120 ects.
- Master in de biochemie en de biotechnologie (Leuven) 120 ects.
- Courses for Exchange Students Faculty of Science (Leuven)
- Master of Biology (Leuven) 120 ects.
Activities
5.3 ects. Genome, Proteome and Metabolome Analysis: Lectures (B-KUL-G0R70a)
Content
Introduction: Terminology
I. Genome sequencing
- Hierarchical and shotgun sequencing strategies
- de novo versus re-sequencing
- Next-generation sequencing platforms
- Analysis of genome content
II. Transcriptomics
- Hybridisation based methods
- Sequencing-based methods
- PCR-based methods
III. Proteomics
• Protein detection methods
• Separation methods
- Sample preparation
- One and two-dimensiobnal gel electroforesis
- Protein blotting
- Gelfree proteomics: multidimensional chromatografy, peptidomics
- Screening methods of chromatografic fractions: bioassays
• Identification methods
- Edman degradation
- Mass spectrometry
- Coupling of protein separation methods to MS
- Quantitative proteomics: Isotope labelling methods
- Search engines for MS-based protein identification
IV. Interactomics
- Protein-protein interaction mapping
- Interaction databases
- Interaction networks
V. Metabolomics
- Fourrier-Transform mass spectrometry
- Element composition analysis
- Metabolite databases
VI. Bioinformatic methods for data processing in proteomics, transcriptomics and metabolomics
Course material
- Lecture slides
- Pdfs of research papers in Toledo
0.7 ects. Genome, Proteome and Metabolome Analysis: Exercises (B-KUL-G0R71a)
Content
See content of main course
Course material
- Course notes and manual in Toledo
- Free software and web tools
Evaluation
Evaluation: Genome, Proteome and Metabolome Analysis (B-KUL-G2R70a)
Explanation
The exam is closed-book and consists of two parts, which are both in written form.
The first part (40% of the toal score) dealing with genomics, transcriptomics and interactomics consists of open questions. The second part (60% of the total score) dealing with mass spectrometry, proteomics and metabolomics consists of open questions, multiple choice questions and exercises.
The final score is the sum of both partial scores. However, for both parts, a minimal score of 10/20 is necessary. A score less than 10/20 for one of the two parts results in an overall score of ≤ 9/20.